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gcb2763-sup-0001-Supplementarymethods.pdfapplication/PDF285KAppendix S1. Supplementary methods. Table S1. Statistical significance (one-way ANOVA + Tukey HSD test) of differences between glaciers (LW – Lower Wright Glacier, RU – Russell Glacier, JE – John Evans Glacier), incubation temperature (1, 4 and 10 °C) and substrate additions in the basic (B), H2/CO2 amended (H) and acetate amended (A) incubations as seen in Figure 5.
gcb2763-sup-0002-Fig1.tifimage/tif12416KFigure S1. Phylogenetic analysis of bacterial clones from Lower Wright Glacier (LW, in red) and Russell Glacier (RU, in green) subglacial sediments. The maximum likelihood tree was constructed based on 474-base-pair-long 16S rRNA gene sequences obtained using a Type-I methanotroph-specific primer set, and is rooted with the archaeon Methanopyrus kandleri. Bootstrap values (1000 replications) >50 are shown. Scale bar represents 10% sequence divergence. Methano/methylotrophic bacteria are marked in blue, and sequences marked in bold originate from similar cold environments.
gcb2763-sup-0003-Fig2.tifimage/tif4544KFigure S2. Phylogenetic analysis of bacterial clones from Lower Wright Glacier (LW, in red) and Russell Glacier (RU, in green) subglacial sediments. The maximum likelihood tree was constructed based on 518-base-pair-long 16S rRNA gene sequences obtained using a Type-II methanotroph-specific primer set, and is rooted with the archaeon Methanopyrus kandleri. Bootstrap values (1000 replications) >50 are shown. Scale bar represents 10% sequence divergence. Methano/methylotrophic bacteria are marked in blue, and sequences marked in bold originate from similar cold environments.

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