The authors stated that they had no interests which might be perceived as posing a conflict or bias.
Diagnosis of factor VIII deficiency
Article first published online: 16 MAY 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd
Special Issue: State of the Art. XXVIII International Congress of the World Federation of Hemophilia
Volume 14, Issue Supplement s3, pages 76–82, July 2008
How to Cite
VERBRUGGEN, B., MEIJER, P., NOVáKOVA, I. and VAN HEERDE, W. (2008), Diagnosis of factor VIII deficiency. Haemophilia, 14: 76–82. doi: 10.1111/j.1365-2516.2008.01715.x
- Issue published online: 16 MAY 2008
- Article first published online: 16 MAY 2008
- Accepted after revision 15 February 2008
- factor VIII;
Summary. The correct diagnosis of factor VIII deficiency and the assessment of severity of the disease are essential for a patient-tailored treatment strategy. An optimal diagnostic procedure comprises sensitive and specific screening methods and factor VIII activity assays. Different screening reagents show variable characteristics and receiver operator characteristic curves are presented showing the relation between sensitivity and specificity of eleven activated partial thromboplastin time reagents. The details of the three methods for factor VIII activity assay, one-stage and two-stage assay and chromogenic assays, are discussed. The chromogenic assay seems to be more sensitive than the one-stage assay with regard to the detection of severe haemophilia. Discrepant results obtained with one-stage and two-stage assays are reviewed and discussed.