Rapid screening of tissue microarrays for Her-2 fluorescence in situ hybridization testing is an accurate, efficient and economic method of providing an entirely in situ hybridization-based Her-2 testing service
Article first published online: 11 MAR 2009
© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Limited
Volume 54, Issue 4, pages 428–432, March 2009
How to Cite
Faratian, D., Graham, A., Rae, F. and Thomas, J. (2009), Rapid screening of tissue microarrays for Her-2 fluorescence in situ hybridization testing is an accurate, efficient and economic method of providing an entirely in situ hybridization-based Her-2 testing service. Histopathology, 54: 428–432. doi: 10.1111/j.1365-2559.2009.03257.x
- Issue published online: 11 MAR 2009
- Article first published online: 11 MAR 2009
- Date of submission 21 July 2008 Accepted for publication 21 October 2008
- breast cancer;
Aim: Fluorescence in situ hybridization (FISH) testing is the ‘gold standard’ method for Her-2 status assessment in breast cancer patients, yet is only employed in about 30% of tests carried out because of cost and labour considerations. We have previously described tissue microarray (TMA)-based testing to eliminate cost constraints, and now describe a rapid screening approach to reduce time spent testing.
Methods and results: We examined 88 cases of invasive breast cancer on TMAs comparing formal FISH scoring with a rapid screening technique. Each core was screened by two observers and results recorded as positive, equivocal or negative. Each approach was timed. Data were analysed by comparing the rapid screening results with formal counts. Using rapid screening, two-thirds of negative and half the positive cases could be eliminated with 100% accuracy. It took 2 min per observer per case to rapid screen six TMA cores at ×100 magnification. The remaining cases required formal counting, which took no longer than with whole-section techniques.
Conclusion: Rapid screening of TMAs for routine Her-2 FISH testing is safe, economical and time efficient. The technique ensures that all patients receive ‘gold standard’ testing.