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Nuclear translocation of β-catenin and decreased expression of epithelial cadherin in human papillomavirus-positive tonsillar cancer: an early event in human papillomavirus-related tumour progression?

Authors


M Stenner, MD, Department of Otorhinolaryngology, Head and Neck Surgery, School of Medicine, University Hospital of Cologne, Kerpener Strasse 62, 50937 Cologne, Germany. e-mail: markus.stenner@uk-koeln.de

Abstract

Stenner M, Yosef B, Huebbers C U, Preuss S F, Dienes H-P, Speel E-J M, Odenthal M & Klussmann J P

(2011) Histopathology58, 1117–1126

Nuclear translocation of β-catenin and decreased expression of epithelial cadherin in human papillomavirus-positive tonsillar cancer: an early event in human papillomavirus-related tumour progression?

Aims:  High-risk human papillomaviruses (HPVs) constitute an important risk factor for tonsillar cancer. This study describes changes in cell adhesion molecules during metastasis of HPV-related and HPV-unrelated tonsillar carcinomas.

Methods and results:  We examined 48 primary tonsillar carcinoma samples (25 HPV-16 DNA-positive, 23 HPV-16 DNA-negative) and their respective lymph node metastases for their HPV status and for the expression of p16, epithelial cadherin (E-cadherin), β-catenin, and vimentin. A positive HPV-specific polymerase chain reaction finding correlated significantly with p16 overexpression in both primary tumours and their metastases (P < 0.0001 for both). In HPV-unrelated carcinomas, the expression of E-cadherin was significantly lower in metastases than in primary tumours (P < 0.001). In contrast, the expression of nuclear β-catenin was significantly higher in metastases than in primary tumours (P = 0.016). In HPV-related carcinomas, nuclear localization of β-catenin expression was already apparent in primary tumours (P = 0.030). The expression of vimentin significantly correlated with the grading of the primary tumour (P = 0.021).

Conclusions:  Our data indicate that the down-regulation of E-cadherin and the up-regulation of nuclear β-catenin expression might be crucial steps during tumour progression of tonsillar carcinomas, being already present in primary tumours in HPV-driven carcinomas, but becoming apparent in HPV-unrelated tumours later in the process of metastasis.

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