M.S.K. and N.G.C. contributed equally to this work.
Genetic and expressional alterations of CHD genes in gastric and colorectal cancers
Article first published online: 30 MAR 2011
© 2011 Blackwell Publishing Limited
Volume 58, Issue 5, pages 660–668, April 2011
How to Cite
Kim, M. S., Chung, N. G., Kang, M. R., Yoo, N. J. and Lee, S. H. (2011), Genetic and expressional alterations of CHD genes in gastric and colorectal cancers. Histopathology, 58: 660–668. doi: 10.1111/j.1365-2559.2011.03819.x
- Issue published online: 12 APR 2011
- Article first published online: 30 MAR 2011
- Date of submission 26 February 2010 Accepted for publication 12 August 2010
- chromodomain helicase DNA-binding proteins;
- colorectal cancer;
- gastric cancer;
- microsatellite instability;
Kim M S, Chung N G, Kang M R, Yoo N J & Lee S H (2011) Histopathology58, 660–668 Genetic and expressional alterations of CHD genes in gastric and colorectal cancers
Aims: Chromodomain helicase DNA-binding protein (CHD) is a regulator of the chromatin remodelling process. The aim was to determine the CHD1, CHD2, CHD3, CHD4, CHD7, CHD8 and CHD9mutational status of mononucleotide repeats in gastric and colorectal cancers with microsatellite instability (MSI).
Methods and Results: The repeats were determined in 28 gastric cancers (GCs) with high MSI (MSI-H), 45 GCs with low MSI (MSI-L)/stable MSI (MSS), 35 colorectal cancers (CRCs) with MSI-H and 45 CRCs with MSI-L/MSS by single-strand conformation polymorphism analysis. CHD4 and CHD8 expressionwas also examined in GCs and CRCs by immunohistochemistry. CHD1, CHD2, CHD3, CHD4, CHD7, CHD8 and CHD9 mutations were found in five, 19, three, five, seven, 10 and seven cancers, respectively. They were detected in MSI-H cancers, but not in MSI-L/MSS cancers. Loss of CHD4 expression was observed in 56.4% of the GCs and 55.7% of the CRCs, and loss of CHD8 was observed in 35.7% of the GCs and 28.6% of the CRCs. The cancers with CHD4 and CHD8 mutations showed loss of CHD4 and CHD8 expression, respectively.
Conclusions: Frameshift mutation and loss of expression of CHD genes are common in GCs and CRCs with MSI-H.These alterations might contribute to cancer pathogenesis by deregulating CHD-mediated chromatin remodelling.