High incidences of DNA ploidy abnormalities in tongue squamous cell carcinoma of young patients: an international collaborative study
Article first published online: 27 JUN 2011
© 2011 Blackwell Publishing Limited
Volume 58, Issue 7, pages 1127–1135, June 2011
How to Cite
Santos-Silva, A. R., Ribeiro, A. C. P., Soubhia, A. M. P., Miyahara, G. I., Carlos, R., Speight, P. M., Hunter, K. D., Torres-Rendon, A., Vargas, P. A. and Lopes, M. A. (2011), High incidences of DNA ploidy abnormalities in tongue squamous cell carcinoma of young patients: an international collaborative study. Histopathology, 58: 1127–1135. doi: 10.1111/j.1365-2559.2011.03863.x
- Issue published online: 27 JUN 2011
- Article first published online: 27 JUN 2011
- Date of submission 9 June 2010 Accepted for publication 23 November 2010
- DNA-image cytometry;
- squamous cell carcinoma;
- tongue cancer;
- young adults
Santos-Silva A R, Ribeiro A C P, Soubhia A M P, Miyahara G I, Carlos R, Speight P M, Hunter K D, Torres-Rendon A, Vargas P A & Lopes M A (2011) Histopathology 58, 1127–1135 High incidences of DNA ploidy abnormalities in tongue squamous cell carcinoma of young patients: an international collaborative study
Aims: This multi-centre analysis assessed the DNA content of TSCC in 37 young patients (<40 years) and 28 old patients (>50 years) and determined the correlation of DNA ploidy findings with clinicopathological data.
Methods and results: Image cytometry was carried out using an automated cellular imaging system on Feulgen-stained histological sections to obtain high-fidelity DNA histograms. Among young patients, 37.8% were females compared to 18.7% in the older group (P = 0.002). In total, 48.6% patients were non-smokers and 40.5% were non-drinkers compared to 10.7% non-smokers and non-drinkers in the older group (P < 0.0001). TNM, clinical stage of disease and histological grade of differentiation did not differ between groups. Tumour aneuploidy was detected in 86.5% and tetraploidy in 24.3% young patients; this was significantly greater than in the older group where 64.3% were aneuploid (P < 0.0001) and 7.2% tetraploid (P < 0.0001). The mean values of DNA index (DI) and DNA heterogeneity index as well as the percentage of cells with DI exceeding 5N were higher in young patients (P < 0.0001).
Conclusions: Young patients with TSCC represent a distinct clinical entity. The high incidence of DNA ploidy abnormalities suggest that they may have increased genomic instability and indicates underlying genetic differences between TSCC in young and older patients.