Expression and significance of stem cell markers in pulmonary sclerosing haemangioma
Article first published online: 13 JUN 2012
© 2012 Blackwell Publishing Ltd
Volume 61, Issue 2, pages 178–185, August 2012
How to Cite
Lin, X.-Y., Fan, C.-F., Dong, X.-J., Yu, J.-H., Dai, S.-D. and Wang, E.-H. (2012), Expression and significance of stem cell markers in pulmonary sclerosing haemangioma. Histopathology, 61: 178–185. doi: 10.1111/j.1365-2559.2011.04088.x
- Issue published online: 30 JUL 2012
- Article first published online: 13 JUN 2012
- Date of submission 16 November 2010 Accepted for publication 4 Febuary 2011
- laser capture microdissection;
- notch signalling pathway;
- pulmonary sclerosing haemangioma;
- stem cell markers
Lin X-Y, Fan C-F, Dong X-J, Yu J-H, Dai S-D & Wang E-H (2012) Histopathology 61, 178–185 Expression and significance of stem cell markers in pulmonary sclerosing haemangioma
Aims: The two major types of cells of pulmonary sclerosing haemangioma (PSH) with the same origin show significant differences in morphological phenotype. Whether these differences are caused by their different differentiation status is still uncertain. The aim of this study was to analyse their differentiation status by detecting the expression of several stem cell markers in PSH.
Methods and results: The expression of stem cell markers was examined by using streptavidin peroxidase (SP) immunohistochemisty in 45 PSH specimens. Also, the two types of cells were, respectively, captured by laser capture microdissection (LCM) from 28 PSH specimens, and total RNA was then extracted followed by reverse transcription–polymerase chain reaction (RT–PCR). The results demonstrated that the expression rates of ABCG2, Notch1 and Notch3 in polygonal cells were significantly higher than those in cuboidal cells (P < 0.05), and the expression levels of ABCG2, Notch3 and Jagged1 in polygonal cells were clearly higher than those in cuboidal cells (P < 0.05).
Conclusion: The data obtained provided evidence that the two types of cells in PSH may be different in differentiation status. The differentiation difference between the two types of cells might lead to variation in their morphological phenotype.