Heterogeneity of lymphoid tissue inducer cell populations present in embryonic and adult mouse lymphoid tissues

Authors


M-Y. Kim, MRC Centre for Immune Regulation, Institute for Biomedical Research, Birmingham Medical School, Birmingham B15 2TT, United Kingdom.
Email: kimmy@ssu.ac.kr
Senior author: Peter J.L. Lane, email: p.j.l.lane@bham.ac.uk

Summary

Lymphoid tissue inducer (LTi) cells have a well established role in secondary lymphoid tissue development. Here, we report on the heterogeneity of LTi cells based on their CD4 and chemokine receptor expression. The CD4 LTi-cell population has a similar phenotype to the CD4+ population, with similar chemokine-receptor-expressing subsets. In both embryonic and adult spleen the CD4 LTi-cell population is comparable as a proportion of total splenocytes to its CD4+ counterpart. In contrast, different proportions of CD4+ and CD4 LTi cells are found in different lymph nodes. Both CD4+ and CD4 LTi cells share the anatomical location and are associated with vascular cell adhesion molecule-1-positive stromal cells in spleen and lymph nodes. The numbers of both CD4+ and CD4 LTi cells in adult spleen are augmented in the presence of B cells. With the exception of CD4, there is a strong correlation coefficient (0·89) for gene expression between the two populations. Polymerase chain reaction analysis of individual CD4+ and CD4 LTi cells shows that a similar proportion in embryonic and adult spleen co-expressed both CXCR5 and CCR7 or CXCR5 alone: 84·6% for adult CD4+ and 87·6% for adult CD4; 95·3% for embryonic CD4+ and 91·5% for embryonic CD4. Consistently fewer CCR7 single-positive cells were found in the CD4+ and CD4 fractions in the embryo.

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