Enhanced secretion of interferon-γ by bovine γδ T cells induced by coculture with Mycobacterium bovis-infected dendritic cells: evidence for reciprocal activating signals
Article first published online: 24 JUL 2008
© 2008 The Authors Journal compilation © 2008 Blackwell Publishing Ltd
Volume 126, Issue 2, pages 201–208, February 2009
How to Cite
Price, S. J. and Hope, J. C. (2009), Enhanced secretion of interferon-γ by bovine γδ T cells induced by coculture with Mycobacterium bovis-infected dendritic cells: evidence for reciprocal activating signals. Immunology, 126: 201–208. doi: 10.1111/j.1365-2567.2008.02889.x
- Issue published online: 5 JAN 2009
- Article first published online: 24 JUL 2008
- Received 17 March 2008; revised 13, 19 May 2008; accepted 21 May 2008.
- γδ T cell;
- major histocompatibility complex class II;
- Mycobacterium bovis
Evidence suggests that γδ T cells form part of the innate immune response to Mycobacterium bovis infection. Dendritic cells (DCs) are capable of secreting high levels of interleukin-12 (IL-12) following infection with mycobacteria and can induce interferon-γ (IFN-γ) secretion by natural killer and γδ T cells We investigated the innate interactions occurring between WC1+γδ T cells and M. bovis-infected DCs. Following coculture with M. bovis-infected DCs, secretion of IFN-γ and expression of CD25 and major histocompatibility complex class II on WC1+γδ T cells were significantly enhanced. Reciprocal enhancement of IL-12 secretion by the DCs was also observed and this interaction was found to be contact dependent. We hypothesize that there is an early, transient signal between the WC1+γδ T cells and the DCs, which promotes the synthesis of biologically active IL-12, and which is dependent upon cell–cell contact. Reciprocal signals including IL-12 are then delivered to WC1+γδ cells, which leads to the enhanced secretion of IFN-γ, and the up-regulation of activation markers and antigen presentation molecules by the WC1+γδ T cells. These interactions are likely to form a critical part of the T helper type 1-conditioning response of DCs to M. bovis.