Expansion of CD4+ CD25+ Foxp3+ T cells by bone marrow-derived dendritic cells
Article first published online: 5 SEP 2008
© 2008 Blackwell Publishing Ltd
Volume 127, Issue 1, pages 50–61, May 2009
How to Cite
Marguti, I., Yamamoto, G. L., da Costa, T. B., Rizzo, L. V. and de Moraes, L. V. (2009), Expansion of CD4+ CD25+ Foxp3+ T cells by bone marrow-derived dendritic cells. Immunology, 127: 50–61. doi: 10.1111/j.1365-2567.2008.02927.x
- Issue published online: 31 MAR 2009
- Article first published online: 5 SEP 2008
- Received 12 March 2008; revised 1 May 2008, 29 May 2008, 16 July 2008; accepted 22 July 2008.
- apoptotic cells;
- dendritic cells;
- regulatory T cells;
Dendritic cells (DCs) are the most important antigen-presenting cells of the immune system and have a crucial role in T-lymphocyte activation and adaptive immunity initiation. However, DCs have also been implicated in maintaining immunological tolerance. In this study, we evaluated changes in the CD4+ CD25+ Foxp3+ T-cell population after co-culture of lymph node cells from BALB/c mice with syngeneic bone marrow-derived DCs. Our results showed an increase in CD4+ CD25+ Foxp3+ T cells after co-culture which occurred regardless of the activation state of DCs and the presence of allogeneic apoptotic cells; however, it was greater when DCs were immature and were pulsed with the alloantigen. Interestingly, syngeneic apoptotic thymocytes were not as efficient as allogeneic apoptotic cells in expanding the CD4+ CD25+ Foxp3+ T-cell population. In all experimental settings, DCs produced high amounts of transforming growth factor (TGF)-β. The presence of allogeneic apoptotic cells induced interleukin (IL)-2 production in immature and mature DC cultures. This cytokine was also detected in the supernatants under all experimental conditions and enhanced when immature DCs were pulsed with the alloantigen. CD4+ CD25+ Foxp3+ T-cell expansion during co-culture of lymph node cells with DCs strongly suggested that the presence of alloantigen enhanced the number of regulatory T cells (Tregs) in vitro. Our data also suggest a role for both TGF-β and IL-2 in the augmentation of the CD4+ CD25+ Foxp3+ population.