A chromatic explosion: the development and future of multiparameter flow cytometry
Article first published online: 28 OCT 2008
© 2008 Blackwell Publishing Ltd. No claim to original US government works
Volume 125, Issue 4, pages 441–449, December 2008
How to Cite
Chattopadhyay, P. K., Hogerkorp, C.-M. and Roederer, M. (2008), A chromatic explosion: the development and future of multiparameter flow cytometry. Immunology, 125: 441–449. doi: 10.1111/j.1365-2567.2008.02989.x
- Issue published online: 5 NOV 2008
- Article first published online: 28 OCT 2008
- Received 23 September 2008; revised 2 October 2008; accepted 7 October 2008.
- flow cytometry;
- T cells;
- data analysis
Multiparameter flow cytometry has matured tremendously since the 1990s, giving rise to a technology that allows us to study the immune system in unprecedented detail. In this article, we review the development of hardware, reagents, and data analysis tools for multiparameter flow cytometry and discuss future advances in the field. Finally, we highlight new applications that use this technology to reveal previously unappreciated aspects of cell biology and immunity.