A chromatic explosion: the development and future of multiparameter flow cytometry

Authors

  • Pratip K. Chattopadhyay,

    1. ImmunoTechnology Section, Laboratory of Immunology, Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
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  • Carl-Magnus Hogerkorp,

    1. ImmunoTechnology Section, Laboratory of Immunology, Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
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  • Mario Roederer

    1. ImmunoTechnology Section, Laboratory of Immunology, Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
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P. K. Chattopadhyay, ImmunoTechnology Section, Laboratory of Immunology, Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Dr., Room 5612 (Bldg. 40), Bethesda, MD 20892-3015, USA. Tel.: (301) 594-8656
Fax: (301) 480-2788 Email: pchattop@mail.nih.gov
Senior author: M. Roederer, email: roederer@mail.nih.gov

Summary

Multiparameter flow cytometry has matured tremendously since the 1990s, giving rise to a technology that allows us to study the immune system in unprecedented detail. In this article, we review the development of hardware, reagents, and data analysis tools for multiparameter flow cytometry and discuss future advances in the field. Finally, we highlight new applications that use this technology to reveal previously unappreciated aspects of cell biology and immunity.

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