• 1
    UNAIDS: Report on the Global AIDS epidemic. [accessed on 26 May 2007]. 2006.
  • 2
    Prakash M, Patterson S, Kapembwa MS. Evaluation of the cervical cytobrush sampling technique for the preparation of CD45+ mononuclear cells from the human cervix. J Immunol Methods 2001; 258:3746.
  • 3
    Addo MM, Yu XG, Rathod A et al. Comprehensive epitope analysis of human immunodeficiency virus type 1 (HIV-1) -specific T-cell responses directed against the entire expressed HIV-1 genome demonstrate broadly directed responses, but no correlation to viral load. J Virol 2003; 77:208192.
  • 4
    Betts MR, Ambrozak DR, Douek DC, Bonhoeffer S, Brenchley JM, Casazza JP, Koup RA, Picker LJ. Analysis of total human immunodeficiency virus (HIV)-specific CD4± and CD8± T-cell responses: relationship to viral load in untreated HIV infection. J Virol 2001; 75:1198391.
  • 5
    Edwards BH, Bansal A, Sabbaj S, Bakari J, Mulligan MJ, Goepfert PA. Magnitude of functional CD8± T-cell responses to the gag protein of HIV-1 correlates inversely with viral load in plasma. J Virol 2002; 76:2298305.
  • 6
    Kaufmann DE, Bailey PM, Sidney J et al. Comprehensive analysis of human immunodeficiency virus type 1-specific CD4 responses reveals marked immunodominance of gag and nef and the presence of broadly recognized peptides. J Virol 2004; 78: 446377.
  • 7
    Ramduth D, Chetty P, Mngquandaniso NC et al. Differential immunogenicity of HIV-1 clade C proteins in eliciting CD8± and CD4± cell responses. J Infect Dis 2005; 192:158896.
  • 8
    Musey L, Ding Y, Cao J, Lee J, Galloway C, Yuen A, Jerome KR, McElrath MJ. Ontogeny and specificities of mucosal and blood human immunodeficiency virus type 1-specific CD8(+) cytotoxic T lymphocytes. J Virol 2003; 77:291300.
  • 9
    Ibarrondo FJ, Anton PA, Fuerst M et al. Parallel human immunodeficiency virus type 1-specific CD8+ T-lymphocyte responses in blood and mucosa during chronic infection. J Virol 2005; 79:428997.
  • 10
    Kaul R, Plummer FA, Kimani J et al. HIV-1-specific mucosal CD8+ lymphocyte responses in the cervix of HIV-1-resistant prostitutes in Nairobi. J Immunol 2000; 164:160211.
  • 11
    Kaul R, Thottingal P, Kimani J, Kiama P, Waigwa CW, Bwayo JJ, Plummer FA, Rowland-Jones SL. Quantitative ex vivo analysis of functional virus-specific CD8 T lymphocytes in the blood and genital tract of HIV-infected women. AIDS 2003; 17:113944.
  • 12
    Shacklett BL, Cu-Uvin S, Beadle TJ et al. Quantification of HIV-1-specific T-cell responses at the mucosal cervicovaginal surface. AIDS 2000; 14:19115.
  • 13
    Gumbi PP, Nkwanyana NN, Bere A et al. Impact of mucosal inflammation on cervical HIV-1-specific CD8 T cell responses in the female genital tract during chronic HIV infection. J Virol 2008; 82:852936.
  • 14
    Sheth PM, Danesh A, Shahabi K et al. HIV-specific CD8+ lymphocytes in semen are not associated with reduced HIV shedding. J Immunol 2005; 175:478996.
  • 15
    Anton PA, Elliott J, Poles MA et al. Enhanced levels of functional HIV-1 co-receptors on human mucosal T cells demonstrated using intestinal biopsy tissue. AIDS 2000; 14:17615.
  • 16
    Guadalupe M, Reay E, Sankaran S, Prindiville T, Flamm J, McNeil A, Dandekar S. Severe CD4+ T-cell depletion in gut lymphoid tissue during primary human immunodeficiency virus type 1 infection and substantial delay in restoration following highly active antiretroviral therapy. J Virol 2003; 77:1170817.
  • 17
    Shacklett BL, Yang O, Hausner MA et al. Optimization of methods to assess human mucosal T-cell responses to HIV infection. J Immunol Methods 2003; 279:1731.
  • 18
    Critchfield JW, Lemongello D, Walker DH, Garcia JC, Asmuth DM, Pollard RB, Shacklett BL. Multifunctional human immunodeficiency virus (HIV) gag-specific CD8+ T-cell responses in rectal mucosa and peripheral blood mononuclear cells during chronic HIV type 1 infection. J Virol 2007; 81:546071.
  • 19
    Musey L, Hu Y, Eckert L, Christensen M, Karchmer T, McElrath MJ. HIV-1 induces cytotoxic T lymphocytes in the cervix of infected women. J Exp Med 1997; 185:293303.
  • 20
    Passmore JS, Milner M, Denny L et al. Mucosal T cell responses to human papillomavirus (HPV) type 16 in women with HPV-associated cervical intraepithelial neoplasia (CIN). Immunology 2006; 119:50714.
  • 21
    Burns GF, Boyd AW, Beverley PC. Two monoclonal anti-human T lymphocyte antibodies have similar biologic effects and recognize the same cell surface antigen. J Immunol 1982; 129:14517.
  • 22
    Nkwanyana NN, Gumbi PP, Roberts L et al. Impact of HIV-1 infection and inflammation on composition and yield of cervical mononuclear cells in the female genital tract. Immunology 2009; 128:e74657.
  • 23
    Jones N, Agrawal D, Elrefaei M, Hanson A, Novitsky V, Wong JT, Cao H. Evaluation of antigen-specific responses using in vitro enriched T cells. J Immunol Methods 2003; 274:13947.
  • 24
    Chen HW, Liao CH, Ying C, Chang CJ, Lin CM. Ex vivo expansion of dendritic-cell-activated antigen-specific CD4+ T cells with anti-CD3/CD28, interleukin-7, and interleukin-15: potential for adoptive T cell immunotherapy. Clin Immunol. 2006; 119:2131.
  • 25
    Kalamasz D, Long SA, Taniguchi R, Buckner JH, Berenson RJ, Bonyhadi M. Optimization of human T-cell expansion ex vivo using magnetic beads conjugated with anti-CD3 and anti-CD28 antibodies. J Immunother 2004; 27:40518.
  • 26
    Keating SM, Bejon P, Berthoud T et al. Durable human memory T cells quantifiable by cultured enzyme-linked immunospot assays are induced by heterologous prime boost immunization and correlate with protection against malaria. J Immunol 2005; 175:567580.
  • 27
    Geginat J, Sallusto F, Lanzavecchia A. Cytokine-driven proliferation and differentiation of human naive, central memory and effector memory CD4+ T cells. Pathol Biol (Paris). 2003; 51:646.
  • 28
    Cohn JA, Gagnon S, Spence MR et al. The role of human papillomavirus deoxyribonucleic acid assay and repeated cervical cytologic examination in the detection of cervical intraepithelial neoplasia among human immunodeficiency virus-infected women. Cervical Disease Study Group Of The American Foundation For Aids Research Community Based Clinical Trials Network. Am J Obstet Gynecol 2001; 184:32230.
  • 29
    Iqbal SM, Ball TB, Kimani J, Kiama P, Thottingal P, Embree JE, Fowke KR, Plummer FA. Elevated T cell counts and RANTES expression in the genital mucosa of HIV-1-resistant Kenyan commercial sex workers. J Infect Dis 2005; 192:72838.
  • 30
    White HD, Crassi KM, Givan AL, Stern JE, Gonzalez JL, Memoli VA, Green WR, Wira CR. CD3+ CD8+ CTL activity within the human female reproductive tract: influence of stage of the menstrual cycle and menopause. J Immunol 1997; 158:301727.