Dissecting T-cell activation with high-resolution live-cell microscopy
Version of Record online: 24 JAN 2012
© 2011 The Authors. Immunology © 2011 Blackwell Publishing Ltd
Volume 135, Issue 3, pages 198–206, March 2012
How to Cite
Illingworth, J. J. and van der Merwe, P. A. (2012), Dissecting T-cell activation with high-resolution live-cell microscopy. Immunology, 135: 198–206. doi: 10.1111/j.1365-2567.2011.03537.x
- Issue online: 24 JAN 2012
- Version of Record online: 24 JAN 2012
- Accepted manuscript online: 10 NOV 2011 12:04PM EST
- Received 23 August 2011; revised 4 November 2011; accepted 8 November 2011.
- immune synapse;
- photoactivated localization microscopy;
- stochastic optical reconstruction microscopy;
- two-colour coincidence detection
Results from live-cell microscopy suggest that the behaviour of isolated components of the T-cell activation machinery in vitro does not represent the reality inside cells. Understanding the cellular-scale dynamics of microcluster migration can only be accomplished by in situ observation. Developments in ‘super-resolution’ microscopy have permitted investigators to move beyond tracking the movements of individual molecules, allowing the recognition of protein islands and nanodomains present in quiescent and active T cells. Many high-resolution techniques have their own susceptibilities to artefacts, so it is important to take a multifaceted approach to confirm results. A major challenge for the future will be to integrate all the new information into a coherent model of antigen recognition and T-cell activation.