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Differential intestinal M-cell gene expression response to gut commensals
Article first published online: 1 JUN 2012
Published 2012. This article is a U.S. Government work and is in the public domain in the USA.
Volume 136, Issue 3, pages 312–324, July 2012
How to Cite
Lapthorne, S., MacSharry, J., Scully, P., Nally, K. and Shanahan, F. (2012), Differential intestinal M-cell gene expression response to gut commensals. Immunology, 136: 312–324. doi: 10.1111/j.1365-2567.2012.03581.x
- Issue published online: 1 JUN 2012
- Article first published online: 1 JUN 2012
- Accepted manuscript online: 3 MAR 2012 01:37PM EST
- Received 14 October 2011; revised 26 January 2012; accepted 15 February 2012.
Figure S1. Evaluation of PRR expression in non polarised C2, polarised C2 and polarised C2-M epithelia-I, CD302 (a), CD302 (b), NLRP3 (c) NLRP11 (D), NOD1(e), NLRC5 (f), CLEC4A (g) and MYD88 (h) expression was measured by qRT-PCR.
Figure S2. Evaluation of PRR expression in non polarised C2, polarised C2 and polarised C2-M epithelia-II, RIPK2 (a), TLR1 (b), TLR2 (c) TLR3 (d), TLR5 (e), TLR6 (f), TLR7 (g) and TLR8 (h) expression was measured by qRT-PCR.
Figure S3. Commensal bacteria induce CCL20 and CLDN4 gene expression in polarised C2 cells.
Figure S4. Co-localisation and translocation of commensal bacteria in murine Peyer’s patch M cells.
Figure S5. Pathway analysis of gene expression profiles of C2-M cells incubated with commensal bacteria.
Figure S6. The effect of commensal bacteria on gene expression of microarray identified gene candidates in polarised C2 cells.
Table S1. List of PCR primers and probes used in this study.
Table S2. List of PCR primers used in the PRR gene expression screen used in this study.
Table S3. List of genes present in each data set corresponding to Fig. 2.
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