Polymerase chain reaction-based identification of insecticide resistance genes and DNA methylation in the aphid Myzus persicae (Sulzer)
Article first published online: 18 DEC 2007
Insect Molecular Biology
Volume 5, Issue 3, pages 197–202, August 1996
How to Cite
Field, L. M., Crlck, S. E. and Devonshire, A. L. (1996), Polymerase chain reaction-based identification of insecticide resistance genes and DNA methylation in the aphid Myzus persicae (Sulzer). Insect Molecular Biology, 5: 197–202. doi: 10.1111/j.1365-2583.1996.tb00054.x
- Issue published online: 18 DEC 2007
- Article first published online: 18 DEC 2007
- Received 15 November 1995; accepted 29 January 1996.
- Myzus persicae;
The ability of a peach-potato aphid (Myzus persicae) to resist insecticides can depend on both the presence of amplified esterase genes and on differences in their expression. Here we report a polymerase chain reaction-restriction enzyme (PCR-REN) technique which can detect the presence of ampiifled esterase genes and distinguish between the two possible types of amplified gene (E4 and FE4) and a homologous fragment from susceptible aphids. The technique is quick and sensitive enough to be used on a fraction of an aphid or an individual embryo. Furthermore, it can be combined with a pre-PCR digestion using a methylation-sensitive enzyme (Hpail) to determine whether or not the esterase genes contain 5–methyicytosine, the presence or absence of which is known to correlate with changes in gene expression.