These two authors contributed equally to this work.
Gene expression of ecdysteroid-regulated gene E74 of the honeybee in ovary and brain
Article first published online: 21 JAN 2005
Insect Molecular Biology
Volume 14, Issue 1, pages 9–15, January 2005
How to Cite
Paul, R. K., Takeuchi, H., Matsuo, Y. and Kubo, T. (2005), Gene expression of ecdysteroid-regulated gene E74 of the honeybee in ovary and brain. Insect Molecular Biology, 14: 9–15. doi: 10.1111/j.1365-2583.2004.00524.x
- Issue published online: 21 JAN 2005
- Article first published online: 21 JAN 2005
- doi: 10.1111/j.1365-2583.2004.00524.x; Received 7 April 2004; accepted after revision 27 July 2004.
- mushroom body;
To facilitate studies of hormonal control in the honeybee (Apis mellifera L.), a cDNA for a honeybee homologue of the ecdysteroid-regulated gene E74 (AmE74) was isolated and its expression was analysed. Northern blot analysis indicated strong expression in the adult queen abdomen, and no significant expression in the adult drone and worker abdomens. In situ hybridization demonstrated that this gene was expressed selectively in the ovary and gut in the queen abdomen. Furthermore, this gene was also expressed selectively in subsets of mushroom body interneurones in the brain of the adult worker bees. These findings suggest that AmE74 is involved in neural function as well as in reproduction in adult honeybees.