Molecular cloning and characterization of two digestive serine proteases from the Hessian fly, Mayetiola destructor
Article first published online: 31 MAY 2005
Insect Molecular Biology
Volume 14, Issue 3, pages 309–318, June 2005
How to Cite
Mittapalli, O., Stuart, J. J. and Shukle, R. H. (2005), Molecular cloning and characterization of two digestive serine proteases from the Hessian fly, Mayetiola destructor. Insect Molecular Biology, 14: 309–318. doi: 10.1111/j.1365-2583.2005.00561.x
- Issue published online: 31 MAY 2005
- Article first published online: 31 MAY 2005
- doi: 10.1111/j.1365-2583.2005.00561.x; Received 18 August 2004; accepted after revision 3 January 2005.
- Hessian fly;
- Mayetiola destructor;
- digestive serine protease;
Full-length cDNA and genomic sequences for two genes (designated mdesprot-I and mdesprot-II) encoding digestive serine proteases in Hessian fly, Mayetiola destructor, have been cloned and characterized. The deduced amino acid sequences revealed similarity with trypsin-like digestive serine proteases from other Dipterans. Both mdesprot-I and mdesprot-II encoded proteins with secretion signal peptides at the N-terminals, indicating the proteins are secreted proteases that should function as midgut digestive proteases. A cytological analysis with fluorescent in situ hybridization revealed the cytological localization of mdesprot-I and mdesprot-II on the long arm of Autosome 2. Results are discussed in the context of the efficacy of potential protease inhibitors to develop Hessian fly resistant wheat through genetic engineering approaches.