The ecdysteroidogenic P450 Cyp302a1/disembodied from the silkworm, Bombyx mori, is transcriptionally regulated by prothoracicotropic hormone

Authors

  • R. Niwa,

    Corresponding author
    1. Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha, Kashiwa, Chiba, Japan,
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      These three authors contributed equally to this work

  • T. Sakudoh,

    1. Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha, Kashiwa, Chiba, Japan,
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      These three authors contributed equally to this work

  • T. Namiki,

    1. Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha, Kashiwa, Chiba, Japan,
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    • *

      These three authors contributed equally to this work

  • K. Saida,

    1. Department of Chemistry and Materials Science, Tokyo Institute of Technology, Meguro, Tokyo, Japan
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  • Y. Fujimoto,

    1. Department of Chemistry and Materials Science, Tokyo Institute of Technology, Meguro, Tokyo, Japan
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  • H. Kataoka

    Corresponding author
    1. Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha, Kashiwa, Chiba, Japan,
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  • doi: 10.1111/j.1365-2583.2005.00587.x

Ryusuke Niwa, Department of Molecular, Cellular and Developmental Biology, Yale University, KBT 938, PO Box 208103, 266 Whitney Ave., New Haven, CT 06520, USA. Tel.: +1 203 432 3493; fax: +1 203 432 3597; e-mail: ryusuke-niwa@umin.ac.jp; and Hiroshi Kataoka, Department of Integrated Biosciences, Rm. 201, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277–8562, Japan. Tel.: +81 47 136 3622; fax: +81 47 136 3623; e-mail: kataoka@k.u-tokyo.ac.jp

Abstract

During larval and pupal development of insects, ecdysone is synthesized in the prothoracic gland (PG). Although several Drosophila genes, including Halloween P450 genes, are known to be important for ecdysteroidogenesis in PG, little is known of the ecdysteroidogenic genes in other insects. Here we report on Cyp302a1/disembodied (dib-Bm), one of the Halloween P450s in the silkworm Bombyx mori that is a carbon-22 hydroxylase. dib-Bm is predominantly expressed in PG and its developmental expression profile is correlated with a change in the ecdysteroid titre in the haemolymph. Furthermore, dib-Bm expression in cultured PGs is significantly induced by treatment with prothoracicotropic hormone. This is the first report on the transcriptional induction of a steroidogenic gene by the tropic hormone in insects.

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