Present address: Natural Health Products Directorate (NHPD), Health Canada, Ottawa, Canada.
The effect of a synergistic concentration of a Piper nigrum extract used in conjunction with pyrethrum upon gene expression in Drosophila melanogaster
Article first published online: 31 MAY 2006
Insect Molecular Biology
Volume 15, Issue 3, pages 329–339, June 2006
How to Cite
Jensen, H. R., Scott, I. M., Sims, S. R., Trudeau, V. L. and Arnason, J. T. (2006), The effect of a synergistic concentration of a Piper nigrum extract used in conjunction with pyrethrum upon gene expression in Drosophila melanogaster. Insect Molecular Biology, 15: 329–339. doi: 10.1111/j.1365-2583.2006.00648.x
- Issue published online: 31 MAY 2006
- Article first published online: 31 MAY 2006
- doi: 10.1111/j.1365-2583.2006.00648.x; Received 07 September 2005; accepted after revision 01 February 2006.
Vol. 15, Issue 6, 861, Article first published online: 13 DEC 2006
- Piper nigrum;
- Drosophila melanogaster;
- DNA microarray;
An ethyl acetate extract of Piper nigrum L. (Piperaceae) peppercorns was tested as a synergist for the botanical insecticide pyrethrum. A high synergist ratio of 11.6 against Drosophila melanogaster was obtained for the combination of pyrethrum supplemented with P. nigrum. The effect of this combination was investigated using cDNA microarray analysis of gene expression profiles in D. melanogaster. Treatment of D. melanogaster with pyrethrum alone resulted in a large number of differentially expressed genes, principally associated with stress responses. Seven genes were identified as being commonly expressed in D. melanogaster treated with at least two of the following treatments: P. nigrum, pyrethrum or P. nigrum plus pyrethrum. These are likely implicated in Drosophila defence responses to toxins.