A new Minos vector for eye-specific expression of white+ marker in Ceratitis capitata and in distantly related dipteran species
Article first published online: 31 MAY 2006
Insect Molecular Biology
Volume 15, Issue 3, pages 341–349, June 2006
How to Cite
Salvemini, M., Mauro, U., Velaeti, S., Polito, C. and Saccone, G. (2006), A new Minos vector for eye-specific expression of white+ marker in Ceratitis capitata and in distantly related dipteran species. Insect Molecular Biology, 15: 341–349. doi: 10.1111/j.1365-2583.2006.00651.x
- Issue published online: 31 MAY 2006
- Article first published online: 31 MAY 2006
- doi: 10.1111/j.1365-2583.2006.00651.x; Received 10 November 2005; accepted after revision 15 February 2006.
- Glass binding sites;
- Ceratitis capitata
The genetic transformation of insects by transposable elements is based on the use of selectable genetic markers required to identify transgenic individuals. Conserved regulatory sequences can be used to develop single constructs capable of adequate expression of a marker, across a range of different species. We present evidence that the Drosophila GBS regulatory element (Glass-binding site), derived from the Rh1 rhodopsin gene, is able to drive in vivo eye-specific expression of a Ccwhite+ transgene in the Mediterranean fruitfly Ceratitis capitata. The Ceratitis lineage diverged from that of Drosophila∼120 Myr ago. As the GBS regulatory sequence seems to be partially conserved in the more distantly related dipteran species Anopheles gambiae (250 Myr), we propose that the GBS may be widely useful for driving eye-specific expression in a wide range of dipteran species.