Transcriptional response of Choristoneura fumiferana to sublethal exposure of Cry1Ab protoxin from Bacillus thuringiensis
Article first published online: 2 AUG 2006
Insect Molecular Biology
Volume 15, Issue 4, pages 475–483, August 2006
How to Cite
Meunier, L., Préfontaine, G., Van Munster, M., Brousseau, R. and Masson, L. (2006), Transcriptional response of Choristoneura fumiferana to sublethal exposure of Cry1Ab protoxin from Bacillus thuringiensis. Insect Molecular Biology, 15: 475–483. doi: 10.1111/j.1365-2583.2006.00659.x
- Issue published online: 2 AUG 2006
- Article first published online: 2 AUG 2006
- doi: 10.1111/j.1365-2583.2006.00659.x; Received 2 December 2005; accepted after revision 16 March 2006.
- Bacillus thuringiensis;
- Choristoneura fumiferana;
- subtractive hybridization;
- gene expression
Bacillus thuringiensis is a microbial control agent active against Choristoneura fumiferana, a lepidopteran defoliator of North American forests. Although the B. thuringiensis insecticidal crystal protoxins have a relatively narrow host range, there is concern about their impact on non-target species where intoxication effects may not be overt. Larval toxicity effects can be assessed at the molecular level by determining altered transcriptional profiles in response to sublethal protoxin exposure in sensitive insects. Subtraction hybridization libraries were created using two larval populations, control and protoxin-fed and were characterized by sequencing 1091 clones. Differential mRNA expression of selected clones, as measured by quantitative polymerase chain reaction, identified a number of metabolic and stress-related genes that were either transcriptionally enhanced or repressed after protoxin exposure.