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Genomic and transcriptional analysis of protein heterogeneity of the honeybee venom allergen Api m 6
Version of Record online: 27 OCT 2006
Insect Molecular Biology
Volume 15, Issue 5, pages 577–581, October 2006
How to Cite
Peiren, N., De Graaf, D. C., Evans, J. D. and Jacobs, F. J. (2006), Genomic and transcriptional analysis of protein heterogeneity of the honeybee venom allergen Api m 6. Insect Molecular Biology, 15: 577–581. doi: 10.1111/j.1365-2583.2006.00669.x
- Issue online: 27 OCT 2006
- Version of Record online: 27 OCT 2006
- Received 15 March 2006; accepted after revision 10 May 2006.
- allelic variation;
- Api m 6;
- bee venom;
- signal peptide
Several components of honeybee venom are known to cause allergenic responses in humans and other vertebrates. One such component, the minor allergen Api m 6, has been known to show amino acid variation but the genetic mechanism for this variation is unknown. Here we show that Api m 6 is derived from a single locus, and that substantial protein-level variation has a simple genome-level cause, without the need to invoke multiple loci or alternatively spliced exons. Api m 6 sits near a misassembled section of the honeybee genome sequence, and we propose that a substantial number of indels at and near Api m 6 might be the root cause of this misassembly. We suggest that genes such as Api m 6 with coding-region or untranslated region indels might have had a strong effect on the assembly of this draft of the honeybee genome.