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X-linked QTL for knockdown resistance to high temperature in Drosophila melanogaster

Authors

  • F. M. Norry,

    1. Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires. (C-1428-EHA) Buenos Aires, Argentina; and * Department of Ecology and Genetics, University of Aarhus, Ny Munkegade, Bldg. 540, DK-8000 Aarhus C, Denmark
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  • P. Sambucetti,

    1. Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires. (C-1428-EHA) Buenos Aires, Argentina; and * Department of Ecology and Genetics, University of Aarhus, Ny Munkegade, Bldg. 540, DK-8000 Aarhus C, Denmark
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  • A. C. Scannapieco,

    1. Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires. (C-1428-EHA) Buenos Aires, Argentina; and * Department of Ecology and Genetics, University of Aarhus, Ny Munkegade, Bldg. 540, DK-8000 Aarhus C, Denmark
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  • F. H. Gomez,

    1. Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires. (C-1428-EHA) Buenos Aires, Argentina; and * Department of Ecology and Genetics, University of Aarhus, Ny Munkegade, Bldg. 540, DK-8000 Aarhus C, Denmark
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  • and * V. Loeschcke

    1. Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires. (C-1428-EHA) Buenos Aires, Argentina; and * Department of Ecology and Genetics, University of Aarhus, Ny Munkegade, Bldg. 540, DK-8000 Aarhus C, Denmark
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Fabian M. Norry, PhD, Departamento de Ecolog’a, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires. (C-1428-EHA) Buenos Aires, Argentina. Tel.: +54(11)45763349/+54(11)43624548; fax: +54(11)45763384; e-mail: fnorry@ege.fcen.uba.ar

Abstract

Knockdown Resistance to High Temperature (KRHT) is an adaptive trait of thermotolerance in insects. An interval mapping was performed on chromosome X of Drosophila melanogaster to search for quantitative trait loci (QTL) affecting KRHT. A backcross population was obtained from two lines that dramatically differ for KRHT. Microsatellites were used as markers. Composite interval mapping identified a large-effect QTL in the region of band 10 where putative candidate genes map. To further test for this QTL a set of recombinant (but non-inbred) lines was obtained from backcrosses between the parental lines used for the interval mapping. Recombinant line analysis confirmed that one QTL is targeted by band 10. We identify and discuss candidate loci contained within our QTL region.

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