Present address: Department of Entomology, Iowa State University, Ames, IA, USA.
Profiling infection responses in the haemocytes of the mosquito, Aedes aegypti
Article first published online: 17 DEC 2007
Insect Molecular Biology
Volume 16, Issue 6, pages 761–776, December 2007
How to Cite
Bartholomay, L. C., Mayhew, G. F., Fuchs, J. F., Rocheleau, T. A., Erickson, S. M., Aliota, M. T. and Christensen, B. M. (2007), Profiling infection responses in the haemocytes of the mosquito, Aedes aegypti. Insect Molecular Biology, 16: 761–776. doi: 10.1111/j.1365-2583.2007.00773.x
- Issue published online: 17 DEC 2007
- Article first published online: 17 DEC 2007
- Received 22 June 2007; accepted after revision 10 September 2007.
Pathogens that infect and/or are transmitted by mosquitoes typically are exposed to the body cavity, and to haemocytes circulating therein, during development or dissemination. Aedes aegypti haemocytes produce a range of immune response-related gene products, and an endpoint response of phagocytosis and/or melanization that is temporally and structurally distinct for the invading pathogen. Expressed sequence tags were generated from haemocyte libraries and then used to design oligonucleotide microarrays. Arrays were screened with haemocyte material collected 1-, 8- and 24-h post-inoculation with Escherichia coli or Micrococcus luteus bacteria. Data from these studies support the discovery of novel immune response-activated genes, provide an expanded understanding of antimicrobial peptide biology and highlight the coordination of immune factors that leads to an endpoint response.