Expression of a mutated phospholipase A2 in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development
Article first published online: 14 MAR 2008
© 2008 The Authors
Insect Molecular Biology
Volume 17, Issue 2, pages 175–183, April 2008
How to Cite
Rodrigues, F. G., Santos, M. N., De Carvalho, T. X. T., Rocha, B. C., Riehle, M. A., Pimenta, P. F. P., Abraham, E. G., Jacobs-Lorena, M., Alves de Brito, C. F. and Moreira, L. A. (2008), Expression of a mutated phospholipase A2 in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development. Insect Molecular Biology, 17: 175–183. doi: 10.1111/j.1365-2583.2008.00791.x
- Issue published online: 14 MAR 2008
- Article first published online: 14 MAR 2008
- Received 30 August 2007; accepted after revision 19 December 2007.
- bee venom phospholipase A2;
The genetic manipulation of mosquito vectors is an alternative strategy in the fight against malaria. It was previously shown that bee venom phospholipase A2 (PLA2) inhibits ookinete invasion of the mosquito midgut although mosquito fitness was reduced. To maintain the PLA2 blocking ability without compromising mosquito biology, we mutated the protein-coding sequence to inactivate the enzyme while maintaining the protein's structure. DNA encoding the mutated PLA2 (mPLA2) was placed downstream of a mosquito midgut-specific promoter (Anopheles gambiae peritrophin protein 1 promoter, AgPer1) and this construct used to transform Aedes fluviatilis mosquitoes. Four different transgenic lines were obtained and characterized and all lines significantly inhibited Plasmodium gallinaceum oocyst development (up to 68% fewer oocysts). No fitness cost was observed when this mosquito species expressed the mPLA2.