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Molecular cloning and expression patterns of a putative olfactory diacylglycerol kinase from the noctuid moth Spodoptera littoralis

Authors

  • B. Chouquet,

    1. UMR 1272, UPMC-INRA-AgroParisTech, Physiologie de l’Insecte: Signalisation et Communication, *Université Paris VI, Paris, France; and †INRA, Versailles Cedex, France
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  • * F. Bozzolan,

    1. UMR 1272, UPMC-INRA-AgroParisTech, Physiologie de l’Insecte: Signalisation et Communication, *Université Paris VI, Paris, France; and †INRA, Versailles Cedex, France
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  • * M. Solvar,

    1. UMR 1272, UPMC-INRA-AgroParisTech, Physiologie de l’Insecte: Signalisation et Communication, *Université Paris VI, Paris, France; and †INRA, Versailles Cedex, France
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  • * L. Duportets,

    1. UMR 1272, UPMC-INRA-AgroParisTech, Physiologie de l’Insecte: Signalisation et Communication, *Université Paris VI, Paris, France; and †INRA, Versailles Cedex, France
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  • * E. Jacquin-Joly,

    1. UMR 1272, UPMC-INRA-AgroParisTech, Physiologie de l’Insecte: Signalisation et Communication, *Université Paris VI, Paris, France; and †INRA, Versailles Cedex, France
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  • P. Lucas,

    1. UMR 1272, UPMC-INRA-AgroParisTech, Physiologie de l’Insecte: Signalisation et Communication, *Université Paris VI, Paris, France; and †INRA, Versailles Cedex, France
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  • and * S. Debernard

    1. UMR 1272, UPMC-INRA-AgroParisTech, Physiologie de l’Insecte: Signalisation et Communication, *Université Paris VI, Paris, France; and †INRA, Versailles Cedex, France
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Stéphane Debernard, Université Paris VI, 7 quai Saint Bernard, Bâtiment A, F-75005 Paris, France. Tel.: +33 1 4427 3839; fax: +33 1 4427 6509; e-mail: stephane.debernard@snv.jussieu.fr

Abstract

In the aim of the characterization of the molecular actors of insect olfactory transduction, we have cloned the full cDNA encoding a Spodoptera littoralis diacylglycerol kinase (DGK) named SlDGK. In male adults, SlDGK transcript was detected predominantly in the brain and in the olfactory sensilla trichodea located on the antennae. SlDGK expression was first detected at day 3 of the pupal stage, then reached a maximum at the end of this stage and was maintained at this level during the adult period. These data provide the first molecular characterization of a DGK potentially involved in the regulation of signalling pathways responsible for the establishment and/or the functioning of the olfactory system in Lepidoptera.

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