Current addresses: University of Bourgogne, Dijon, France; and †Department of Neurobiology, University of Massachusetts Medical School, Worcester, MA, USA.
Molecular identification and characterization of two new Lepidoptera chemoreceptors belonging to the Drosophila melanogaster OR83b family
Version of Record online: 16 SEP 2008
© 2008 The Authors. Journal compilation © 2008 The Royal Entomological Society
Insect Molecular Biology
Volume 17, Issue 5, pages 587–596, October 2008
How to Cite
Malpel, S., Merlin, C., François, M.-C. and Jacquin-Joly, E. (2008), Molecular identification and characterization of two new Lepidoptera chemoreceptors belonging to the Drosophila melanogaster OR83b family. Insect Molecular Biology, 17: 587–596. doi: 10.1111/j.1365-2583.2008.00830.x
- Issue online: 16 SEP 2008
- Version of Record online: 16 SEP 2008
- Received 16 March 2008; accepted after revision 6 June 2008.
- olfactory receptor;
- Mamestra brassicae;
- Spodoptera littoralis
In insect antennae, olfaction depends on olfactory receptors (ORs) that function through heterodimerization with an unusually highly conserved partner orthologue to the Drosophila melanogaster DOR83b. Here, we report the identification of two cDNAs encoding new DOR83b orthologues that represent the first members, although nonconventional, of the OR families of two noctuid crop pests, the cotton leafworm Spodoptera littoralis and the cabbage armyworm Mamestra brassicae. They both displayed high protein sequence conservation with previously identified DOR83b orthologues. Transcripts were abundantly detected in adult chemosensory organs as well as in fifth instar larvae heads. In adult antennae, the expression patterns of both genes revealed common features with other members of the OR83b subfamily: they appeared to be expressed at the bases of numerous olfactory sensilla belonging to different functional categories, suggesting that both receptors may be co-expressed with yet unidentified conventional ORs. Bioinformatic analyses predicted the occurrence of seven transmembrane domains and an unusual topology with intracellular N-termini and extracellular C-termini, extending to Lepidoptera the hypothesis of an inverted topology for DOR83b orthologues, demonstrated to date only in D. melanogaster.