Wounding-mediated gene expression and accelerated viviparous reproduction of the pea aphid Acyrthosiphon pisum
Article first published online: 24 SEP 2008
© 2008 The Authors. Journal compilation © 2008 The Royal Entomological Society
Insect Molecular Biology
Volume 17, Issue 6, pages 711–716, December 2008
How to Cite
Altincicek, B., Gross, J. and Vilcinskas, A. (2008), Wounding-mediated gene expression and accelerated viviparous reproduction of the pea aphid Acyrthosiphon pisum. Insect Molecular Biology, 17: 711–716. doi: 10.1111/j.1365-2583.2008.00835.x
- Issue published online: 6 NOV 2008
- Article first published online: 24 SEP 2008
- Received 14 May 2008; accepted after revision 2 July 2008.
- evolutionary ecology;
- insect immunity;
Most insects mount a potent antimicrobial defence upon contact with microbes or microbe-associated pattern molecules. Using a combined set of methods for analysis of insect innate immunity, we report here that piercing of the pea aphid Acyrthosiphon pisum with a bacteria-contaminated needle elicits lysozyme-like activity in the haemolymph but no detectable activities against live bacteria. Confirming these results, we found no homologues of known antimicrobial peptides in our cDNA library generated by using the suppression subtractive hybridization method or in over 90 000 public expressed sequence tag (EST) sequences, but lysozyme genes have recently been described in A. pisum. Interestingly, we discovered that production of viviparous offspring was significantly accelerated upon wounding. Therefore, we postulate that aphids may increase terminal reproductive investment and limit antibacterial defence in response to a threat to their survival.