Chikungunya-Wolbachia interplay in Aedes albopictus

Authors

  • P. Tortosa,

    1. Université Montpellierz 2;
    2. CNRS, Institut des Sciences de l’Evolution, Equipe Génétique de l’Adaptation, C.C. 065, Place Eugène Bataillon 34095 Montpellier cedex 05, France; and
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  • A. Courtiol,

    1. Université Montpellierz 2;
    2. CNRS, Institut des Sciences de l’Evolution, Equipe Génétique de l’Adaptation, C.C. 065, Place Eugène Bataillon 34095 Montpellier cedex 05, France; and
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  • S. Moutailler,

    1. Institut Pasteur, Unité de Génétique moléculaire des Bunyavirus, 25-28 rue du Docteur Roux, 75724 Paris, France
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  • A.-B. Failloux,

    1. Institut Pasteur, Unité de Génétique moléculaire des Bunyavirus, 25-28 rue du Docteur Roux, 75724 Paris, France
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  • M. Weill

    1. Université Montpellierz 2;
    2. CNRS, Institut des Sciences de l’Evolution, Equipe Génétique de l’Adaptation, C.C. 065, Place Eugène Bataillon 34095 Montpellier cedex 05, France; and
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M. Weill, CNRS, Institut des Sciences de l’Evolution, Equipe Génétique de l’Adaptation, C.C. 065, Place Eugène Bataillon 34095 Montpellier cedex 05, France. Tel.: +33 4 67 14 32 62; Fax: +33 4 67 14 36 22; E-mail: mylene.weill@univ-montp2.fr

Abstract

A severe Chikungunya (CHIK) outbreak recently hit several countries of the Indian Ocean. On La Réunion Island, Aedes albopictus was incriminated as the major vector. This mosquito species is naturally co-infected with two distinct strains of the endosymbiont Wolbachia, namely wAlbA and wAlbB, which are increasingly attracting interest as potential tools for vector control. A PCR quantitative assay was developed to investigate Wolbachia/mosquito host interactions. We show that Wolbachia densities are slightly decreased in CHIK virus (CHIKV)-infected females. We measured the impact of CHIKV replication on a lysogenic virus: WO bacteriophage. Our data indicate that WO is sheltered by wAlbB, likely at a single copy per bacteria, and that CHIKV replication is not a physiological stress triggering WO entrance into the lytic cycle.

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