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A TRP channel is expressed in Spodoptera littoralis antennae and is potentially involved in insect olfactory transduction

Authors

  • B. Chouquet,

    1. UMR 1272 Physiologie de l’Insecte: Signalisation et Communication, Université Pierre et Marie Curie, Paris VI, F-75005 Paris, France; and
    2. INRA, route de Saint-Cyr, F-78000 Versailles, France
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  • S. Debernard,

    1. UMR 1272 Physiologie de l’Insecte: Signalisation et Communication, Université Pierre et Marie Curie, Paris VI, F-75005 Paris, France; and
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  • F. Bozzolan,

    1. UMR 1272 Physiologie de l’Insecte: Signalisation et Communication, Université Pierre et Marie Curie, Paris VI, F-75005 Paris, France; and
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  • M. Solvar,

    1. UMR 1272 Physiologie de l’Insecte: Signalisation et Communication, Université Pierre et Marie Curie, Paris VI, F-75005 Paris, France; and
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  • M. Maïbèche-Coisné,

    1. UMR 1272 Physiologie de l’Insecte: Signalisation et Communication, Université Pierre et Marie Curie, Paris VI, F-75005 Paris, France; and
    2. INRA, route de Saint-Cyr, F-78000 Versailles, France
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  • P. Lucas

    1. INRA, route de Saint-Cyr, F-78000 Versailles, France
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  • B. Chouquet, S. Debernard have contributed equally to this work.

Philippe Lucas, UMR 1272 Physiologie de l’Insecte: Signalisation et Communication,INRA, route de Saint-Cyr, F-78000 Versailles, France. Tel.: +33-1-30 83 37 37; fax: +33-1-30 83 31 19; e-mail: plucas@versailles.inra.fr

Abstract

The molecular characterization of post-receptor actors involved in insect olfactory transduction has yet to be understood. We have investigated the presence of a Transient Receptor Potential (TRP) channel in the peripheral olfactory system of the moth Spodoptera littoralis. A cDNA encoding a Lepidopteran TRP channel (TRPγ) was identified by analysis of a male-antennal EST database and subsequently cloned by RACE PCR. In adult males, the TRPγ transcript was detected in antennae, at the base of olfactory sensilla. Moreover, TRPγ was observed in antennae in both pupal and adult stages. This work is the first step in understanding the involvement of TRPγ in signalling pathways involved in the development and function of the insect olfactory system.

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