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Identification of the gene encoding pro-phenoloxidase A3 in the fruitfly, Drosophila melanogaster
Article first published online: 9 JAN 2009
DOI: 10.1111/j.1365-2583.2008.00858.x
© 2009 The Authors. Journal compilation © 2009 The Royal Entomological Society
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How to Cite
Asano, T. and Takebuchi, K. (2009), Identification of the gene encoding pro-phenoloxidase A3 in the fruitfly, Drosophila melanogaster. Insect Molecular Biology, 18: 223–232. doi: 10.1111/j.1365-2583.2008.00858.x
Publication History
- Issue published online: 13 MAR 2009
- Article first published online: 9 JAN 2009
- Abstract
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Keywords:
- pro-phenoloxidase;
- phenoloxidase;
- insect immunity;
- melanin formation;
- activation;
- Drosophila melanogaster
Abstract
The fruitfly, Drosophila melanogaster, has three proPO genes (DoxA1, CG8193 and CG2952). DoxA1 has been shown to encode proPO A1, one of the two proPO isoforms (A1 and A3). However, which of CG8193 or CG2952 encodes proPO A3 has so far remained elusive. In Northern analysis, CG8193 expression was strong during the larval stage, yet expression of CG2952 was not detected at any stage. Immunoblot analyses with specific antibodies detected CG8193 in the larval hemolymph at the mobility of the endogenous proPO A3, though no signal for CG2952. These results indicate that the expression of CG2952 is very low and that CG8193 is the gene that encodes proPO A3. Processing of A1 and A3 isoforms in adult homogenate and activity of recombinant proPOs were also investigated.