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Figure S1. Detection of Cecropin B1, Attacin and Lysozyme mRNAs by Northern blotting. Total RNA was extracted from the Bombyx mori fat bodies excised from larvae 8 h after immunization and analysed by Northern blotting. RP49 mRNAs were analysed as an internal control. Three samples were examined for each condition. (A) Comparison of mRNA levels after stimulation by saline (control), crude lipopolysaccharide (cLPS: 0.1 or 1.0 µg/larva), and TLRgradeTM LPS (pLPS: 0.1 or 1.0 µg/larva) from Salmonella abortus equi. (B) Comparison of mRNA levels after stimulation by saline (control), cLPS (1.0 µg/larva), and pLPS (1.0 µg/larva) from S. abortus equi (Sa) and Escherichia coli (Ec). (C) Comparison of mRNA levels after stimulation by lipid A (1.8 µg/larva) or dimethyl sulphoxide (DMSO; control).

Figure S2. Induction of Cecropin B1 gene expression by crude lipopolysaccharide (cLPS) and peptidoglycans (PGNs), but not by TLRgradeTM LPS, in NIAS-Bm-aff3 cells. cLPS (0.01, 0.1, and 1.0 µg/ml), TLRgradeTM LPS (pLPS: 0.01, 0.1 and 1.0 µg/ml) from Salmonella abortus equi (Sa) and PGN from Escherichia coli (0.01, 0.1 and 1.0 µg/ml) were incubated with NIAS-Bm-aff3 cells for 8 h and total RNA was extracted from the cells. RP49 mRNAs were analysed as an internal control.

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