Present addresses: Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain; and §Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA.
Identification and characterization of the doublesex gene of Nasonia
Version of Record online: 23 MAR 2009
© 2009 The Authors. Journal compilation © 2009 The Royal Entomological Society
Insect Molecular Biology
Volume 18, Issue 3, pages 315–324, June 2009
How to Cite
Oliveira, D. C. S. G., Werren, J. H., Verhulst, E. C., Giebel, J. D., Kamping, A., Beukeboom, L. W. and Van De Zande, L. (2009), Identification and characterization of the doublesex gene of Nasonia. Insect Molecular Biology, 18: 315–324. doi: 10.1111/j.1365-2583.2009.00874.x
- Issue online: 15 MAY 2009
- Version of Record online: 23 MAR 2009
- DM domain genes;
- sex determination
The doublesex (dsx) gene of the parasitic wasp Nasonia vitripennis is described and characterized. Differential splicing of dsx transcripts has been shown to induce somatic sexual differentiation in Diptera and Lepidoptera, but not yet in other insect orders. Two spliceforms of Nasonia dsx mRNA are differentially expressed in males and females. In addition, in a gynandromorphic line that produces haploids (normally males) with full female phenotypes, these individuals show the female spliceform, providing the first demonstration of a direct association of dsx with somatic sex differentiation in Hymenoptera. Finally, the DNA binding (DM) domain of Nasonia dsx clusters phylogenetically with dsx from other insects, and Nasonia dsx shows microsynteny with dsx of Apis, further supporting identification of the dsx orthologue in Nasonia.