Sugar transporters of the major facilitator superfamily in aphids; from gene prediction to functional characterization
Article first published online: 23 FEB 2010
© 2010 The Authors. Journal compilation © 2010 The Royal Entomological Society
Insect Molecular Biology
Special Issue: The Aphid Genome
Volume 19, Issue Supplement s2, pages 97–112, March 2010
How to Cite
Price, D. R. G., Tibbles, K., Shigenobu, S., Smertenko, A., Russell, C. W., Douglas, A. E., Fitches, E., Gatehouse, A. M. R. and Gatehouse, J. A. (2010), Sugar transporters of the major facilitator superfamily in aphids; from gene prediction to functional characterization. Insect Molecular Biology, 19: 97–112. doi: 10.1111/j.1365-2583.2009.00918.x
- Issue published online: 23 FEB 2010
- Article first published online: 23 FEB 2010
Figure S1. Sequence similarity tree (Maximum Likelihood method) of sugar transporters containing the IPR003663 signature in Acyrthosiphon pisum (Blue), Drosophila melanogaster (Red), Apis mellifera (Brown) and other insects (Purple). For each branch, the posterior probability is shown. The scale bar indicates an evolutionary distance of 0.4 amino acid substitutions per position in the sequence.
Table S1. Survey of genes encoding proteins belonging to clan MFS (Pfam CL0015) in the genome of Acyrthosiphon pisum, and assignment to different families within the clan. ‘Predicted genes’ = total number of different potential genes identified; ‘Reference sequences’ = predicted genes for which consistent evidence in the form of predictions from different methods and complete open reading frames available. Most ‘reference sequences’ also have corresponding expressed sequence tags. Sequences identified by BLAST analysis, cut off = 0.01. (Numbers of predicted genes differ from detailed analysis due to manual correction of duplication, incorrect assignments, etc.; note that many Sugar_tr genes are included in MFS_1).
Table S2. Summary of genes predicted to encode proteins belonging to Pfam Sugar_tr family (PF00083) in Acyrthosiphon pisum. Additional genes encoding synaptic vesicle proteins and genes in family MFS_1 which are similar to organic cation transporters are included. ‘ST_ID’ (Ap_ST) numbers are temporary designations for protein products, which will be replaced by approved gene symbols. Interpro ID indicates the presence of signatures diagnostic for sugar transporters from the InterPro database. ‘Most similar Drosophila protein’ indicates best orthologue in the Drosophila melanogaster genome.
Table S3. Numbers of predicted genes encoding putative sugar transporters in different species. Predicted proteins from genome sequence data were scanned for matches using the Interpro IPR003663 sugar/inositol transporter signature, and the Pfam PF00083 Sugar_tr family motif. The scan was carried out using the HMMER program, with cut-off for matching set to e-value < 1.0e-10.
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