First published online 22 October 2009.
Abundant nuclear copies of mitochondrial origin (NUMTs) in the Aedes aegypti genome
Article first published online: 22 OCT 2009
© 2009 The Authors. Journal compilation © 2009 The Royal Entomological Society
Insect Molecular Biology
Volume 18, Issue 6, pages 705–713, December 2009
How to Cite
Black IV, W. C. and Bernhardt, S. A. (2009), Abundant nuclear copies of mitochondrial origin (NUMTs) in the Aedes aegypti genome. Insect Molecular Biology, 18: 705–713. doi: 10.1111/j.1365-2583.2009.00925.x
- Issue published online: 8 NOV 2009
- Article first published online: 22 OCT 2009
- Aedes aegypti;
- mitochondrial insertions;
A portion of the Aedes aegypti mitochondrial NADH dehydrogenase subunit 4 gene (ND4) was amplified using PCR with a 42 °C annealing temperature. Amplified fragments from individual mosquitoes were similar to ND4 but contained multiple segregating sites. We suspected that nuclear copies of mitochondrial origin (NUMTs) exist in the Ae. aegypti genome. A BlastN search in VectorBase with the entire Ae. aegypti mitochondrial genome identified 233 NUMTs comprising 110 178 bp in 145 supercontigs. At a density of 0.080 bp/kb, this represents the second highest density of NUMTs in an insect genome and the highest in Diptera. Analyses of flanking sequences suggested that Ae. aegypti NUMTs arise through mtDNA leakage from damaged mitochondria followed by breakage and nonhomologous recombination, rather than through duplicative processes such as transposition or molecular drive.