Data mining cDNAs reveals three new single stranded RNA viruses in Nasonia (Hymenoptera: Pteromalidae)

Authors


  • Present address: Deodoro C. S. G. Oliveira, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, BCN 08193, Spain.

John W. Werren, 306 Hutchinson Hall, Department of Biology, University of Rochester, Rochester, NY 14627, USA. Tel.: +1 585 275 3889; fax: +1 585 275 2070; e-mail: werr@mail.rochester.edu

Abstract

We report three novel small RNA viruses uncovered from cDNA libraries from parasitoid wasps in the genus Nasonia. The genome of this kind of virus is a positive-sense single-stranded RNA with a 3′ poly(A), which facilitates cloning from cDNAs. Two of the viruses, NvitV-1 and NvitV-2, possess a RNA-dependent RNA polymerase that associates them with the family Iflaviridae of the order Picornavirales. A third virus, NvitV-3, is most similar to the Nora virus from Drosophila. A reverse transcription-PCR method developed for NvitV-1 indicates that it is a persistent commensal infection of Nasonia.

Ancillary