Present address: Deodoro C. S. G. Oliveira, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, BCN 08193, Spain.
Data mining cDNAs reveals three new single stranded RNA viruses in Nasonia (Hymenoptera: Pteromalidae)
Version of Record online: 15 JAN 2010
© 2010 The Authors. Journal compilation © 2010 The Royal Entomological Society
Insect Molecular Biology
Special Issue: The Nasonia Genome
Volume 19, Issue Supplement s1, pages 99–107, February 2010
How to Cite
Oliveira, D. C. S. G., Hunter, W. B., Ng, J., Desjardins, C. A., Dang, P. M. and Werren, J. H. (2010), Data mining cDNAs reveals three new single stranded RNA viruses in Nasonia (Hymenoptera: Pteromalidae). Insect Molecular Biology, 19: 99–107. doi: 10.1111/j.1365-2583.2009.00934.x
- Issue online: 15 JAN 2010
- Version of Record online: 15 JAN 2010
- picorna-like virus;
- insect RNA virus;
- RNA-dependent RNA polymerase
We report three novel small RNA viruses uncovered from cDNA libraries from parasitoid wasps in the genus Nasonia. The genome of this kind of virus is a positive-sense single-stranded RNA with a 3′ poly(A), which facilitates cloning from cDNAs. Two of the viruses, NvitV-1 and NvitV-2, possess a RNA-dependent RNA polymerase that associates them with the family Iflaviridae of the order Picornavirales. A third virus, NvitV-3, is most similar to the Nora virus from Drosophila. A reverse transcription-PCR method developed for NvitV-1 indicates that it is a persistent commensal infection of Nasonia.