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Data mining cDNAs reveals three new single stranded RNA viruses in Nasonia (Hymenoptera: Pteromalidae)

  1. D. C. S. G. Oliveira1,
  2. W. B. Hunter2,
  3. J. Ng1,
  4. C. A. Desjardins1,
  5. P. M. Dang3,
  6. J. H. Werren1,*

Article first published online: 15 JAN 2010

DOI: 10.1111/j.1365-2583.2009.00934.x

Issue

Insect Molecular Biology

Insect Molecular Biology

Special Issue: The Nasonia Genome

Volume 19, Issue Supplement s1, pages 99–107, February 2010

Additional Information

How to Cite

Oliveira, D. C. S. G., Hunter, W. B., Ng, J., Desjardins, C. A., Dang, P. M. and Werren, J. H. (2010), Data mining cDNAs reveals three new single stranded RNA viruses in Nasonia (Hymenoptera: Pteromalidae). Insect Molecular Biology, 19: 99–107. doi: 10.1111/j.1365-2583.2009.00934.x

Author Information

  1. 1

    Department of Biology, University of Rochester, Rochester, NY, USA;

  2. 2

    United States Department of Agriculture, Agricultural Research Service, US Horticultural Research Laboratory, Fort Pierce, FL, USA; and

  3. 3

    United States Department of Agriculture, Agricultural Research Service, NPRU, Dawson, GA, USA

*John W. Werren, 306 Hutchinson Hall, Department of Biology, University of Rochester, Rochester, NY 14627, USA. Tel.: +1 585 275 3889; fax: +1 585 275 2070; e-mail: werr@mail.rochester.edu

  1. Present address: Deodoro C. S. G. Oliveira, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, BCN 08193, Spain.

Publication History

  1. Issue published online: 15 JAN 2010
  2. Article first published online: 15 JAN 2010

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Keywords:

  • picorna-like virus;
  • Picornavirales;
  • insect RNA virus;
  • RNA-dependent RNA polymerase

Abstract

We report three novel small RNA viruses uncovered from cDNA libraries from parasitoid wasps in the genus Nasonia. The genome of this kind of virus is a positive-sense single-stranded RNA with a 3′ poly(A), which facilitates cloning from cDNAs. Two of the viruses, NvitV-1 and NvitV-2, possess a RNA-dependent RNA polymerase that associates them with the family Iflaviridae of the order Picornavirales. A third virus, NvitV-3, is most similar to the Nora virus from Drosophila. A reverse transcription-PCR method developed for NvitV-1 indicates that it is a persistent commensal infection of Nasonia.

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