Present address: Faculty of Fisheries, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki-shi 852-8521, Japan.
A full-length cDNA resource for the pea aphid, Acyrthosiphon pisum
Article first published online: 23 FEB 2010
© 2010 The Authors. Journal compilation © 2010 The Royal Entomological Society
Insect Molecular Biology
Special Issue: The Aphid Genome
Volume 19, Issue Supplement s2, pages 23–31, March 2010
How to Cite
Shigenobu, S., Richards, S., Cree, A. G., Morioka, M., Fukatsu, T., Kudo, T., Miyagishima, S., Gibbs, R. A., Stern, D. L. and Nakabachi, A. (2010), A full-length cDNA resource for the pea aphid, Acyrthosiphon pisum. Insect Molecular Biology, 19: 23–31. doi: 10.1111/j.1365-2583.2009.00946.x
- Issue published online: 23 FEB 2010
- Article first published online: 23 FEB 2010
- full-length cDNA;
- functional genomics;
- EST analysis
Large collections of full-length cDNAs are important resources for genome annotation and functional genomics. We report the creation of a collection of 50 599 full-length cDNA clones from the pea aphid, Acyrthosiphon pisum. Sequencing from 5′ and 3′ ends of the clones generated 97 828 high-quality expressed sequence tags, representing approximately 9000 genes. These sequences were imported to AphidBase and are shown to play crucial roles in both automatic gene prediction and manual annotation. Our detailed analyses demonstrated that the full-length cDNAs can further improve gene models and can even identify novel genes that are not included in the current version of the official gene set. This full-length cDNA collection can be utilized for a wide variety of functional studies, serving as a community resource for the study of the functional genomics of the pea aphid.