A full-length cDNA resource for the pea aphid, Acyrthosiphon pisum


Shuji Shigenobu, Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, Higashiyama, Myodaiji, Okazaki 444-8787, Japan. Tel.: +81 564 59 5875; fax: +81 564 59 5879; e-mail: shige@nibb.ac.jp; Atsushi Nakabachi, Advanced Science Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. Tel.: +81 48 467 9332; fax: +81 48 462 9329; e-mail: bachi@riken.jp


Large collections of full-length cDNAs are important resources for genome annotation and functional genomics. We report the creation of a collection of 50 599 full-length cDNA clones from the pea aphid, Acyrthosiphon pisum. Sequencing from 5′ and 3′ ends of the clones generated 97 828 high-quality expressed sequence tags, representing approximately 9000 genes. These sequences were imported to AphidBase and are shown to play crucial roles in both automatic gene prediction and manual annotation. Our detailed analyses demonstrated that the full-length cDNAs can further improve gene models and can even identify novel genes that are not included in the current version of the official gene set. This full-length cDNA collection can be utilized for a wide variety of functional studies, serving as a community resource for the study of the functional genomics of the pea aphid.