Halloween genes and nuclear receptors in ecdysteroid biosynthesis and signalling in the pea aphid
Version of Record online: 23 FEB 2010
© 2010 The Authors. Journal compilation © 2010 The Royal Entomological Society
Insect Molecular Biology
Special Issue: The Aphid Genome
Volume 19, Issue Supplement s2, pages 187–200, March 2010
How to Cite
Christiaens, O., Iga, M., Velarde, R. A., Rougé, P. and Smagghe, G. (2010), Halloween genes and nuclear receptors in ecdysteroid biosynthesis and signalling in the pea aphid. Insect Molecular Biology, 19: 187–200. doi: 10.1111/j.1365-2583.2009.00957.x
- Issue online: 23 FEB 2010
- Version of Record online: 23 FEB 2010
Figure S1. Amino acid sequence alignment of Spo/Spok/Spot (A), Phm (B), Dib (C) Sad (D) and Shd (E). Residues in black are identities and in gray are similarities. The conserved P450 motifs are indicated: helix-C, helix-I, helix-K, PERF-motif and heme-binding domain.
Figure S2. Amino acid sequences of the cloned and sequenced fragments of EcR (A), Usp (B), E75 (C) and HR3 (D). For EcR and Usp, the entire DBD has been sequenced and the P box (yellow), D box (red) and T/A box (teal) are indicated. The eight zinc-coordinating cysteines in the DBD are boxed in the sequence. For E75, only a part of the DBD has been sequenced.
Table S1. Primers used for the RT-PCR detection in the transcriptome of the Halloween (upper part) and nuclear receptor genes and for the cloning of EcR, Usp, E75 and HR3 (lower part) in the pea aphid. The primers were designed to overlap at least one intron, except for the KNRL and EG genes, which mainly consist out of one big exon and only possess a very small second exon. Expected fragment sizes are also indicated in the table
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