Yellow fever mosquito sterol carrier protein-2 gene structure and transcriptional regulation
Article first published online: 1 DEC 2009
© 2009 The Authors. Journal compilation © 2009 The Royal Entomological Society
Insect Molecular Biology
Volume 19, Issue 2, pages 205–215, April 2010
How to Cite
Vyazunova, I. and Lan, Q. (2010), Yellow fever mosquito sterol carrier protein-2 gene structure and transcriptional regulation. Insect Molecular Biology, 19: 205–215. doi: 10.1111/j.1365-2583.2009.00959.x
- Issue published online: 3 MAR 2010
- Article first published online: 1 DEC 2009
- First published online 1 December 2009.
- sterol carrier protein;
- transcription factor;
- insect hormone
AeSCP-2, a sterol carrier protein, is involved in sterol trafficking in mosquitoes. The activity of the AeSCP-2 gene is important for mosquito development. An earlier study demonstrated that the transcription of this gene was upregulated by 20-hydroxyecdysone (20E) in cultured gut tissues. To investigate 20E-regulated transcription of the AeSCP-2 gene we truncated the upstream flanking region of AeSCP-2 gene and linked it to a reporter gene. The mosquito Aag-2 cell line was transfected with these promoter/reporter constructs and treated with 20E at various concentrations. Expression vectors of different transcription factors such as HR3 and βFTZ-F1 were also co-transfected with the AeSCP-2 promoter/reporter constructs. The observed results demonstrated that varied combinations of transcription factors produce different promoter activities of the AeSCP-2 gene. This observation leads us to the conclusion that the partnership of transcription factors is crucial in regulating the transcriptional activity of the AeSCP-2 gene.