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Development of bacterial artificial chromosome library resources for parasitoid Hymenoptera (Nasonia vitripennis and Nasonia giraulti: Pteromalidae)

  1. M. C. Muñoz-Torres1,*,
  2. C. Saski1,
  3. B. Blackmon1,
  4. J. Romero-Severson2,
  5. J. H. Werren3,†

Article first published online: 15 JAN 2010

DOI: 10.1111/j.1365-2583.2009.00962.x

Issue

Insect Molecular Biology

Insect Molecular Biology

Special Issue: The Nasonia Genome

Volume 19, Issue Supplement s1, pages 181–187, February 2010

Additional Information

How to Cite

Muñoz-Torres, M. C., Saski, C., Blackmon, B., Romero-Severson, J. and Werren, J. H. (2010), Development of bacterial artificial chromosome library resources for parasitoid Hymenoptera (Nasonia vitripennis and Nasonia giraulti: Pteromalidae). Insect Molecular Biology, 19: 181–187. doi: 10.1111/j.1365-2583.2009.00962.x

Author Information

  1. 1

    Clemson University Genomics Institute, Clemson, SC, USA;

  2. 2

    Department of Biological Sciences, University of Notre Dame, South Bend, IN, USA; and

  3. 3

    Department of Biological Sciences, University of Rochester, Rochester, NY, USA

  1. †

    contact for BAC library resources and data repository.

*Mónica C. Muñoz-Torres, Department of Biology, Georgetown University, Washington, DC 20057, USA. Tel.: +1 202 687 5855; fax: +1 202 687 5662; e-mail: mcm262@georgetown.edu

  • †

    contact for BAC library resources and data repository.

Publication History

  1. Issue published online: 15 JAN 2010
  2. Article first published online: 15 JAN 2010

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Keywords:

  • Nasonia;
  • parasitoid;
  • genomics;
  • fingerprinting;
  • BAC

Abstract

The species of the genus Nasonia possess qualities that make them excellent candidates for genetic and genomic studies. To increase the wealth of genomic resources for the genus we constructed publicly available bacterial artificial chromosome (BAC) libraries for Nasonia vitripennis and Nasonia giraulti. Libraries have 36 864 clones each, empty-vector contents of ∼2% and average insert sizes of 113.1 and 97.7 Kb, respectively, representing 12 and 11 genome equivalents. The N. vitripennis library was used for genome sequence assembly and in efforts at positional cloning of a developmental gene. The genome assembly of N. vitripennis is currently composed on 6181 un-joined scaffolds. These BAC libraries can be used to identify and close regions between scaffolds of the genome assemblies of both species.

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