Present address: C. M. Grozinger, Department of Entomology, Center for Chemical Ecology, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA.
The effects of mating and instrumental insemination on queen honey bee flight behaviour and gene expression
Article first published online: 11 DEC 2009
© 2009 The Authors. Journal compilation © 2009 The Royal Entomological Society
Insect Molecular Biology
Volume 19, Issue 2, pages 153–162, April 2010
How to Cite
Kocher, S. D., Tarpy, D. R. and Grozinger, C. M. (2010), The effects of mating and instrumental insemination on queen honey bee flight behaviour and gene expression. Insect Molecular Biology, 19: 153–162. doi: 10.1111/j.1365-2583.2009.00965.x
- Issue published online: 3 MAR 2010
- Article first published online: 11 DEC 2009
- First published online 11 December 2009.
- honey bee;
- gene expression;
- instrumental insemination;
Mating is fundamental to most organisms, although the physiological and transcriptional changes associated with this process have been largely characterized only in Drosophila melanogaster. In this study, we use honey bees as a model system because their queens undergo massive and permanent physiological and behavioural changes following mating. Previous studies have identified changes associated with the transition from a virgin queen to a fully mated, egg-laying queen. Here, we further uncouple the mating process to examine the effects of natural mating vs. instrumental insemination and saline vs. semen insemination. We observed effects on flight behaviour, vitellogenin expression and significant overlap in transcriptional profiles between our study and analogous studies in D. melanogaster, suggesting that some post-mating mechanisms are conserved across insect orders.