Insect intestinal mucins and serine proteases associated with the peritrophic matrix from feeding, starved and moulting Mamestra configurata larvae

Authors

  • U. Toprak,

    1. Molecular Genetics Section, Agriculture and Agri-Food Canada, Saskatoon, SK, Canada;
    2. Department of Biology, University of Saskatchewan, Saskatoon, SK, Canada;
    3. Department of Plant Protection, College of Agriculture, University of Ankara, Turkey; and
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  • D. Baldwin,

    1. Molecular Genetics Section, Agriculture and Agri-Food Canada, Saskatoon, SK, Canada;
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  • M. Erlandson,

    1. Molecular Genetics Section, Agriculture and Agri-Food Canada, Saskatoon, SK, Canada;
    2. Department of Biology, University of Saskatchewan, Saskatoon, SK, Canada;
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  • C. Gillott,

    1. Department of Biology, University of Saskatchewan, Saskatoon, SK, Canada;
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  • D. D. Hegedus

    Corresponding author
    1. Molecular Genetics Section, Agriculture and Agri-Food Canada, Saskatoon, SK, Canada;
    2. Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, SK, Canada
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Dwayne D. Hegedus, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK, Canada S7N 0X2. Tel.: +1 306 956 7667; fax: +1 306 956 7247; e-mail: hegedusd@agr.gc.ca

Abstract

Insect intestinal mucins (McIIM2-4) expressed in the midgut of feeding, starved and moulting Mamestra configurata larvae were identified. McIIM2 and McIIM4 were associated with the peritrophic matrix (PM). PMs from feeding and starved larvae were translucent and contained organized chitin bundles perpendicular to their long axis, whereas PM from moulting larvae consisted of an inner opaque mass surrounded by an outer translucent sleeve. Serine protease genes (McSP1, McSP2, McSP25 and McSP29) were also expressed in these larvae and several serine proteases were associated with the PM. Serine protease activity was also detected in the midgut of feeding, starved and moulting larvae.

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