Transcription profiling of eleven cytochrome P450s potentially involved in xenobiotic metabolism in the mosquito Aedes aegypti


Stéphane Reynaud, Laboratoire d'Ecologie Alpine (LECA), UMR CNRS-Université 5553, Equipe Perturbations Environnementales et Xénobiotiques, Domaine Universitaire de Saint-Martin d'Hères, 2233, rue de la piscine Bât D Biologie, BP 53, 38041 Grenoble Cedex 9, France. Tel.: + 33 0 4 76 51 44 59; fax: + 33 0 4 76 51 44 63; e-mail:


Transcription profiles of 11 Aedes aegypti P450 genes from CYP6 and CYP9 subfamilies potentially involved in xenobiotic metabolism were investigated. Many genes were preferentially transcribed in tissues classically involved in xenobiotic metabolism including midgut and Malpighian tubules. Life-stage transcription profiling revealed important variations amongst larvae, pupae, and adult males and females. Exposure of mosquito larvae to sub-lethal doses of three xenobiotics induced the transcription of several genes with an induction peak after 48 to 72 h exposure. Several CYP genes were also induced by oxidative stress and one gene strongly responded to 20-hydroxyecdysone. Overall, this study revealed that these P450s show different transcription profiles according to xenobiotic exposures, life stages or sex. Their putative chemoprotective functions are discussed.