Sheila M. Dreher-Lesnick and Shane M. Ceraul contributed equally.
Analysis of Rickettsia typhi-infected and uninfected cat flea (Ctenocephalides felis) midgut cDNA libraries: deciphering molecular pathways involved in host response to R. typhi infection
Article first published online: 15 DEC 2009
© 2009 The Authors. Journal compilation © 2009 The Royal Entomological Society
Insect Molecular Biology
Volume 19, Issue 2, pages 229–241, April 2010
How to Cite
Dreher-Lesnick, S. M., Ceraul, S. M., Lesnick, S. C., Gillespie, J. J., Anderson, J. M., Jochim, R. C., Valenzuela, J. G. and Azad, A. F. (2010), Analysis of Rickettsia typhi-infected and uninfected cat flea (Ctenocephalides felis) midgut cDNA libraries: deciphering molecular pathways involved in host response to R. typhi infection. Insect Molecular Biology, 19: 229–241. doi: 10.1111/j.1365-2583.2009.00978.x
- Issue published online: 3 MAR 2010
- Article first published online: 15 DEC 2009
- First published online 15 December 2009.
- R. typhi
Murine typhus is a flea-borne febrile illness that is caused by the obligate intracellular bacterium, Rickettsia typhi. The cat flea, Ctenocephalides felis, acquires R. typhi by imbibing a bloodmeal from a rickettsemic vertebrate host. To explore which transcripts are expressed in the midgut in response to challenge with R. typhi, cDNA libraries of R. typhi-infected and uninfected midguts of C. felis were constructed. In this study, we examined midgut transcript levels for select C. felis serine proteases, GTPases and defence response genes, all thought to be involved in the fleas response to feeding or infection. An increase in gene expression was observed for the serine protease inhibitors and vesicular trafficking proteins in response to feeding. In addition, R. typhi infection resulted in an increase in gene expression for the chymotrypsin and rab5 that we studied. Interestingly, R. typhi infection had little effect on expression of any of the defence response genes that we studied. We are unsure as to the physiological significance of these gene expression profiles and are currently investigating their potential roles as it pertains to R. typhi infection. To our knowledge, this is the first report of differential expression of flea transcripts in response to infection with R. typhi.