The first two authors contributed equally to the report.
Two novel Salp15-like immunosuppressant genes from salivary glands of Ixodes persulcatus Schulze tick
Version of Record online: 26 FEB 2010
© 2010 The Authors. Journal compilation © 2010 The Royal Entomological Society
Insect Molecular Biology
Volume 19, Issue 3, pages 359–365, June 2010
How to Cite
Mori, A., Konnai, S., Yamada, S., Hidano, A., Murase, Y., Ito, T., Takano, A., Kawabata, H., Onuma, M. and Ohashi, K. (2010), Two novel Salp15-like immunosuppressant genes from salivary glands of Ixodes persulcatus Schulze tick. Insect Molecular Biology, 19: 359–365. doi: 10.1111/j.1365-2583.2010.00994.x
- Issue online: 4 MAY 2010
- Version of Record online: 26 FEB 2010
- Ixodes persulcatus;
- Lyme borreliosis
Salp15, a 15-kDa tick salivary gland protein, is known for several suppressive activities against host immunity and critical functions for the transmission of Lyme borrelia in Ixodes scapularis and Ixodes ricinus, the major vectors found in North America and Western Europe. Salp15 inhibits the activation of cluster of differentiation (CD)4+T-cells through the repression of T-cell receptor (TCR)-triggered calcium fluxes and interleukin (IL)-2 production. Furthermore, Salp15 adheres to the spirochaeta and specifically interacts with its outer surface protein C. The binding of Salp15 to Borrelia burgdorferi protects it from antibody-mediated killing in vitro. The aim of this study is to identify the Salp15 genes in Ixodes persulcatus Schulze, the specific vector for human Lyme borreliosis in Japan. Two cDNA clones encoding the Salp15-like sequence were obtained from salivary glands of fed female ticks. These genes encode 135- and 132-amino acid proteins, designated Salp15 Iper-1 and Salp15 Iper-2, respectively, both having signal peptide sequences and predicted to be secretory proteins. Salp15 Iper-1 and -2 showed 51.8 and 68.2% similarity to I. scapularis Salp15, respectively. Reverse transcriptase PCR analysis showed that Salp15 Iper genes were expressed specifically in the salivary glands throughout life cycle stages of the ticks and were up-regulated by blood feeding. In the I. persulcatus-derived sequences, the C-terminal part, which is the binding domain to the CD4 molecule of T-cells in I. scapularis Salp15, was well conserved. In the future, it will be necessary to analyse immunosuppressive functions of I. persulcatus Salp15 and their interaction with Borrelia spp. in Japan.