Present address: Department of Tumor Immunology, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
CREB expression in the brains of two closely related parasitic wasp species that differ in long-term memory formation
Article first published online: 4 MAR 2010
© 2010 The Authors. Journal compilation © 2010 The Royal Entomological Society
Insect Molecular Biology
Volume 19, Issue 3, pages 367–379, June 2010
How to Cite
Van Den Berg, M., Verbaarschot, P., Hontelez, S., Vet, L. E. M., Dicke, M. and Smid, H. M. (2010), CREB expression in the brains of two closely related parasitic wasp species that differ in long-term memory formation. Insect Molecular Biology, 19: 367–379. doi: 10.1111/j.1365-2583.2010.00997.x
Note: the nucleotide data of the splice variants and the predicted amino acid sequence data of the isoforms can be retrieved from GenBank (http://www.ncbi.nlm.nih.gov/Genbank/) under the following accession numbers: FJ155907, FJ155908, FJ155909, FJ155910, FJ155911, FJ155912, FJ155913, FJ155914, FJ155915, FJ155916, FJ155917, FJ155918, FJ155919, FJ155920, FJ155921, FJ155922, FJ155923, and FJ155924.
- Issue published online: 4 MAY 2010
- Article first published online: 4 MAR 2010
- parasitoid wasp;
- Cotesia glomerata;
- Cotesia rubecula
The cAMP/PKA signalling pathway and transcription factor cAMP response element-binding protein (CREB) play key roles in long-term memory (LTM) formation. We used two closely related parasitic wasp species, Cotesia glomerata and Cotesia rubecula, which were previously shown to be different in LTM formation, and sequenced at least nine different CREB transcripts in both wasp species. The splicing patterns, functional domains and amino acid sequences were similar to those found in the CREB genes of other organisms. The predicted amino acid sequences of the CREB isoforms were identical in both wasp species. Using real-time quantitative PCR we found that two low abundant CREB transcripts are differentially expressed in the two wasps, whereas the expression levels of high abundant transcripts are similar.