SEARCH

SEARCH BY CITATION

Figure S1. The localization of the expressed transgene products in the salivary gland from both female monomeric DsRed (mDsRed)-SP15 and tetrameric DsRed (tDsRed) transgenic mosquitoes. 3D confocal imaging of a longitudinal section of distal–lateral lobe stained with SYTO-13, of each transgenic mosquito line. SYTO-13 green-fluorescent dye stains DNA and RNA, facilitated visualization of the shape of the secretory cells. Overlaying the red and green fluorescence images showed that the tDsRed protein was located in the secretory cells and the mDsRed-SP15 protein was located in the secretory cavities and ducts of the salivary glands. C, secretory cell; Sc, salivary cavity; Sd, salivary duct. Scale bars = 100 μm.

Figure S2. Expression of the sp15 gene in the salivary glands and carcasses of nonfusion SP15 (SP15) male and female mosquitoes. The sp15 mRNA specifically expressed in the female SP15 salivary glands but not in the male SP15 or wild-type salivary glands or their carcasses.

Video S1. Video of the physical rupture of the salivary gland in the mDsRed-SP15 mosquito. The experimental procedures are described in the Experimental procedures of Supporting Information.

Video S2. Video of the physical rupture of the salivary gland in the tDsRed mosquito. The experimental procedures are described in the Experimental procedures of Supporting Information.

Video S3. Video of the release of saliva with red fluorescence during salivation from the proboscis of mDsRed-SP15 mosquito (bottom). No red fluorescence was observed in saliva from nontransgenic (wild-type) mosquito (top). The experimental procedures are described in the Experimental procedures of Supporting Information.

Table S1. List of primers.

Please note: Neither the Editors nor Wiley-Blackwell are responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.

FilenameFormatSizeDescription
IMB_1000_sm_Figures_S1-2.pdf1368KSupporting info item
IMB_1000_sm_Videos_S1-3.zip8853KSupporting info item

Please note: Neither the Editors nor Wiley Blackwell are responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.