Present address: Graduate School of Bio-Agricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
Molecular characterization of a gene encoding juvenile hormone esterase in the red flour beetle, Tribolium castaneum
Article first published online: 2 JUN 2010
© 2010 The Authors. Journal compilation © 2010 The Royal Entomological Society
Insect Molecular Biology
Volume 19, Issue 4, pages 527–535, August 2010
How to Cite
Tsubota, T., Minakuchi, C., Nakakura, T., Shinoda, T. and Shiotsuki, T. (2010), Molecular characterization of a gene encoding juvenile hormone esterase in the red flour beetle, Tribolium castaneum. Insect Molecular Biology, 19: 527–535. doi: 10.1111/j.1365-2583.2010.01019.x
- Issue published online: 1 JUL 2010
- Article first published online: 2 JUN 2010
- cDNA cloning;
- juvenile hormone titer regulation;
Juvenile hormone esterases (JHEs) are required for the degradation of juvenile hormones (JHs) in insects. Here, we report the cloning and analysis of the jhe gene in the red flour beetle, Tribolium castaneum, a model insect of Coleoptera. The Tcjhe gene was strongly expressed at the final instar larva, as would be expected if it functioned to decrease the JH titer at this stage. A recombinant TcJHE protein efficiently degraded JH III, suggesting that the enzyme functions in vivo as a JH-specific degradation enzyme. This is the first report describing the developmental expression profile of the jhe gene whose enzymatic activity was shown in Coleoptera, and the new data reported here will aid elucidation of the mechanism of JH titer regulation in insects.